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rabbit polyclonal anti insulin receptor substrate 1 irs1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit polyclonal anti insulin receptor substrate 1 irs1
    Western blot detection of insulin signaling proteins in subcutaneous adipose tissues. These tissues were collected on day 2 postpartum from CON cows ( n = 3) and OVE cows ( n = 3). a Panels of INSR, <t>IRS1,</t> p-IRS1, AKT, p-AKT (Thr308) and p-AKT (Ser473) protein. β-actin was measured as an internal control. b Intensities of INSR, IRS1, p-IRS1, AKT, p-AKT (Thr308) and p-AKT (Ser473) bands were determined using Quantity One software. The results are presented as the ratio of INSR band intensity to the β-actin band intensity, the ratio of p-ISR1 band intensity to IRS1 band intensity, and the ratio of p-AKT (Thr308) and p-AKT (Ser473) band intensities to the AKT band intensity. IRS1, insulin receptor substrate 1; AKT, protein kinase B
    Rabbit Polyclonal Anti Insulin Receptor Substrate 1 Irs1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1404 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+insulin+receptor+substrate+1+irs1/pmc06518689-87-35-51?v=Cell+Signaling+Technology+Inc
    Average 96 stars, based on 1404 article reviews
    rabbit polyclonal anti insulin receptor substrate 1 irs1 - by Bioz Stars, 2026-08
    96/100 stars

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    1) Product Images from "Prepartum body conditions affect insulin signaling pathways in postpartum adipose tissues in transition dairy cows"

    Article Title: Prepartum body conditions affect insulin signaling pathways in postpartum adipose tissues in transition dairy cows

    Journal: Journal of Animal Science and Biotechnology

    doi: 10.1186/s40104-019-0347-4

    Western blot detection of insulin signaling proteins in subcutaneous adipose tissues. These tissues were collected on day 2 postpartum from CON cows ( n = 3) and OVE cows ( n = 3). a Panels of INSR, IRS1, p-IRS1, AKT, p-AKT (Thr308) and p-AKT (Ser473) protein. β-actin was measured as an internal control. b Intensities of INSR, IRS1, p-IRS1, AKT, p-AKT (Thr308) and p-AKT (Ser473) bands were determined using Quantity One software. The results are presented as the ratio of INSR band intensity to the β-actin band intensity, the ratio of p-ISR1 band intensity to IRS1 band intensity, and the ratio of p-AKT (Thr308) and p-AKT (Ser473) band intensities to the AKT band intensity. IRS1, insulin receptor substrate 1; AKT, protein kinase B
    Figure Legend Snippet: Western blot detection of insulin signaling proteins in subcutaneous adipose tissues. These tissues were collected on day 2 postpartum from CON cows ( n = 3) and OVE cows ( n = 3). a Panels of INSR, IRS1, p-IRS1, AKT, p-AKT (Thr308) and p-AKT (Ser473) protein. β-actin was measured as an internal control. b Intensities of INSR, IRS1, p-IRS1, AKT, p-AKT (Thr308) and p-AKT (Ser473) bands were determined using Quantity One software. The results are presented as the ratio of INSR band intensity to the β-actin band intensity, the ratio of p-ISR1 band intensity to IRS1 band intensity, and the ratio of p-AKT (Thr308) and p-AKT (Ser473) band intensities to the AKT band intensity. IRS1, insulin receptor substrate 1; AKT, protein kinase B

    Techniques Used: Western Blot, Control, Software



    Similar Products

    rabbit polyclonal anti insulin receptor substrate 1 irs1  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc rabbit polyclonal anti insulin receptor substrate 1 irs1
    Western blot detection of insulin signaling proteins in subcutaneous adipose tissues. These tissues were collected on day 2 postpartum from CON cows ( n = 3) and OVE cows ( n = 3). a Panels of INSR, <t>IRS1,</t> p-IRS1, AKT, p-AKT (Thr308) and p-AKT (Ser473) protein. β-actin was measured as an internal control. b Intensities of INSR, IRS1, p-IRS1, AKT, p-AKT (Thr308) and p-AKT (Ser473) bands were determined using Quantity One software. The results are presented as the ratio of INSR band intensity to the β-actin band intensity, the ratio of p-ISR1 band intensity to IRS1 band intensity, and the ratio of p-AKT (Thr308) and p-AKT (Ser473) band intensities to the AKT band intensity. IRS1, insulin receptor substrate 1; AKT, protein kinase B
    Rabbit Polyclonal Anti Insulin Receptor Substrate 1 Irs1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+insulin+receptor+substrate+1+irs1/pmc06518689-87-35-51?v=Cell+Signaling+Technology+Inc
    Average 96 stars, based on 1 article reviews
    rabbit polyclonal anti insulin receptor substrate 1 irs1 - by Bioz Stars, 2026-08
    96/100 stars
    		  Buy from Supplier
    rabbit polyclonal anti insulin receptor substrate 1 irs1  (Proteintech)
    95
    Proteintech rabbit polyclonal anti insulin receptor substrate 1 irs1
    Fig. 4 Western blot detection of insulin signaling proteins in subcutaneous adipose tissues. These tissues were collected on day 2 postpartum from CON cows (n = 3) and OVE cows (n = 3). a Panels of INSR, <t>IRS1,</t> p-IRS1, AKT, p-AKT (Thr308) and p-AKT (Ser473) protein. β-actin was measured as an internal control. b Intensities of INSR, IRS1, p-IRS1, AKT, p-AKT (Thr308) and p-AKT (Ser473) bands were determined using Quantity One software. The results are presented as the ratio of INSR band intensity to the β-actin band intensity, the ratio of p-ISR1 band intensity to IRS1 band intensity, and the ratio of p-AKT (Thr308) and p-AKT (Ser473) band intensities to the AKT band intensity. IRS1, insulin receptor substrate 1; AKT, protein kinase B
    Rabbit Polyclonal Anti Insulin Receptor Substrate 1 Irs1, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+insulin+receptor+substrate+1+irs1/pm31114678-77-35-65?v=Proteintech
    Average 95 stars, based on 1 article reviews
    rabbit polyclonal anti insulin receptor substrate 1 irs1 - by Bioz Stars, 2026-08
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    Western blot detection of insulin signaling proteins in subcutaneous adipose tissues. These tissues were collected on day 2 postpartum from CON cows ( n = 3) and OVE cows ( n = 3). a Panels of INSR, IRS1, p-IRS1, AKT, p-AKT (Thr308) and p-AKT (Ser473) protein. β-actin was measured as an internal control. b Intensities of INSR, IRS1, p-IRS1, AKT, p-AKT (Thr308) and p-AKT (Ser473) bands were determined using Quantity One software. The results are presented as the ratio of INSR band intensity to the β-actin band intensity, the ratio of p-ISR1 band intensity to IRS1 band intensity, and the ratio of p-AKT (Thr308) and p-AKT (Ser473) band intensities to the AKT band intensity. IRS1, insulin receptor substrate 1; AKT, protein kinase B

    Journal: Journal of Animal Science and Biotechnology

    Article Title: Prepartum body conditions affect insulin signaling pathways in postpartum adipose tissues in transition dairy cows

    doi: 10.1186/s40104-019-0347-4

    Figure Lengend Snippet: Western blot detection of insulin signaling proteins in subcutaneous adipose tissues. These tissues were collected on day 2 postpartum from CON cows ( n = 3) and OVE cows ( n = 3). a Panels of INSR, IRS1, p-IRS1, AKT, p-AKT (Thr308) and p-AKT (Ser473) protein. β-actin was measured as an internal control. b Intensities of INSR, IRS1, p-IRS1, AKT, p-AKT (Thr308) and p-AKT (Ser473) bands were determined using Quantity One software. The results are presented as the ratio of INSR band intensity to the β-actin band intensity, the ratio of p-ISR1 band intensity to IRS1 band intensity, and the ratio of p-AKT (Thr308) and p-AKT (Ser473) band intensities to the AKT band intensity. IRS1, insulin receptor substrate 1; AKT, protein kinase B

    Article Snippet: The following primary antibodies were used: rabbit polyclonal anti-protein kinase B (AKT) (#9272S; 1:1,000 dilution), rabbit monoclonal anti-phospho(p)-AKT (Ser473) (#9272; 1:2,000 dilution), rabbit monoclonal anti-p-AKT (Thr308) (D25E6; 1:1,000 dilution), mouse monoclonal anti-INSR (ab69508; 1:1,000 dilution), rabbit polyclonal anti-insulin receptor substrate 1 (IRS1) (#2382; 1:1,000 dilution), rabbit polyclonal anti-p-IRS1 (#2381; 1:1,000 dilution) (Cell Signaling Technology, Inc., Danvers, MA, USA), and mouse anti-β-actin Mab (66009–1-Ig; 1:1,000 dilution; Proteintech Group Inc., Chicago, IL, USA).

    Techniques: Western Blot, Control, Software

    Fig. 4 Western blot detection of insulin signaling proteins in subcutaneous adipose tissues. These tissues were collected on day 2 postpartum from CON cows (n = 3) and OVE cows (n = 3). a Panels of INSR, IRS1, p-IRS1, AKT, p-AKT (Thr308) and p-AKT (Ser473) protein. β-actin was measured as an internal control. b Intensities of INSR, IRS1, p-IRS1, AKT, p-AKT (Thr308) and p-AKT (Ser473) bands were determined using Quantity One software. The results are presented as the ratio of INSR band intensity to the β-actin band intensity, the ratio of p-ISR1 band intensity to IRS1 band intensity, and the ratio of p-AKT (Thr308) and p-AKT (Ser473) band intensities to the AKT band intensity. IRS1, insulin receptor substrate 1; AKT, protein kinase B

    Journal: Journal of animal science and biotechnology

    Article Title: Prepartum body conditions affect insulin signaling pathways in postpartum adipose tissues in transition dairy cows.

    doi: 10.1186/s40104-019-0347-4

    Figure Lengend Snippet: Fig. 4 Western blot detection of insulin signaling proteins in subcutaneous adipose tissues. These tissues were collected on day 2 postpartum from CON cows (n = 3) and OVE cows (n = 3). a Panels of INSR, IRS1, p-IRS1, AKT, p-AKT (Thr308) and p-AKT (Ser473) protein. β-actin was measured as an internal control. b Intensities of INSR, IRS1, p-IRS1, AKT, p-AKT (Thr308) and p-AKT (Ser473) bands were determined using Quantity One software. The results are presented as the ratio of INSR band intensity to the β-actin band intensity, the ratio of p-ISR1 band intensity to IRS1 band intensity, and the ratio of p-AKT (Thr308) and p-AKT (Ser473) band intensities to the AKT band intensity. IRS1, insulin receptor substrate 1; AKT, protein kinase B

    Article Snippet: The following primary antibodies were used: rabbit polyclonal anti-protein kinase B (AKT) (#9272S; 1:1,000 dilution), rabbit monoclonal anti-phospho(p)-AKT (Ser473) (#9272; 1:2,000 dilution), rabbit monoclonal anti-p-AKT (Thr308) (D25E6; 1:1,000 dilution), mouse monoclonal anti-INSR (ab69508; 1:1,000 dilution), rabbit polyclonal anti-insulin receptor substrate 1 (IRS1) (#2382; 1:1,000 dilution), rabbit polyclonal anti-p-IRS1 (#2381; 1:1,000 dilution) (Cell Signaling Technology, Inc., Danvers, MA, USA), and mouse anti-β-actin Mab (66009–1-Ig; 1:1,000 dilution; Proteintech Group Inc., Chicago, IL, USA).

    Techniques: Western Blot, Control, Software